A rate - limiting step to long - term survival in heart transplant patients is coronary arteriopathy , which is marked intimal proliferation with lumenal narrowing of the small epicardial and intramyocardial coronary artery branches , leading to ischemic changes 冠狀動脈影響到心臟移植病人能否長期存活,它的特征有內膜增生伴有心外膜以及心肌內的冠狀動脈分支管腔狹窄,從而導致缺血性改變。
Translocation from endocytic compartments to the cytosol is the essential and rate - limiting step in the intoxication process of most toxins such as ricin , diphtheria toxin , shiga toxin and pseudomonas exotoxin ( pe ) . a number of these toxins are transported to trans - golgi network ( tgn ) , and in many cases such transport to the tgn is required for the translocation and cytotoxicity . in deed , 5 % of the ricin endocytosed by cells has been shown to reach the tgn 蓖麻毒素進入細胞的機理不甚明了,一般認為是rtb先與細胞膜受體結合,主要經過受體介導的內吞作用進入吞噬體,然后沿著內體、高爾基體、內質網(wǎng)等逆向分泌途徑,有序地運輸?shù)絻荣|網(wǎng),最后從內質網(wǎng)轉位進入細胞漿,在胞漿內攻擊核糖體,從而抑制蛋白質的合成,導致細胞死亡。
In order to break down the rate - limited steps in the artemisinin biosynthesis to improve the artemisinin production and realize the industrial production of artemisinin , related key genes in artemisinin biosynthesis must be cloned and the regulatory patterns of key genes should be studied . for this purpose molecular cloning of related key genes in artemisinin biosynthesis was performed in this thesis work 利用現(xiàn)代分子生物學和基因工程技術手段,克隆青蒿素生成途徑的關鍵酶基因,研究關鍵酶基因對青蒿素生物合成的調控規(guī)律,是打破青蒿素生物合成的限速步驟,大幅度提高青蒿素含量,最終達到利用植物生物技術工業(yè)化生產青蒿素的目的必須解決的關鍵問題。